New en bloc staining technique improve tissue contrast and electrical conductivity (1050.2)

Large-volume en-bloc staining for electron microscopy-based connectomics

Large-scale connectomics requires dense staining of neuronal tissue blocks for electron microscopy (EM). Here we report a large-volume dense en-bloc EM staining protocol that overcomes the staining gradients, which so far substantially limited the reconstructable volumes in three-dimensional (3D) EM. Our protocol provides densely reconstructable tissue blocks from mouse neocortex sized at least...

A new technique for Gram staining paraffin-embedded tissue.

Five techniques for Gram staining bacteria in paraffin sections were compared on serial sections of pulmonary tissues from eight bacteriological necropsies. Brown and Hopp's method was the most satisfactory for distinguishing Gram-positive and Gram-negative bacteria. However, this method cannot be recommended as the preparations were frequently overstained, and the Gram-negative bacteria were s...

Is the branched graft technique better than the en bloc technique for total aortic arch replacement?

OBJECTIVES Total aortic arch replacement remains a surgical challenge. For the reimplantation of the supra-aortic vessels, either the en bloc (island) or branched graft technique (BGT) is used. The BGT has been proposed to have several advantages over the classical island technique. The purpose of this study was to compare the perioperative and mid-term follow-up results of these two methods. ...

A new staining technique for polar bodies.

Transplantation of infant kidneys – The surgical technique en bloc and transplant position variation: A case report Transplantacija dečjih bubrega – hirurška tehnika en bloc i varijacija pozicije transplantata

Introduction. Due to the ever-present lack of kidney transplant grafts, more and more organs obtained from the so-called “marginal donors” group are accepted, which can provide suboptimal effect of transplantation, depending on their characteristics and/or implantation techniques. Case report. We presented a case with successful variation of kidney position with modified approach of kidney tran...